🔬 5F-AKB48: Uses, Properties & Manufacturing Process

✅ Chemical Name:

N-(1-Amino-3-methyl-1-oxobutan-2-yl)-1-(5-fluoropentyl)-1H-indazole-3-carboxamide

✅ Common Names:

• 5F-AKB48

• 5F-APINACA

• 5F-AKB-48F

• 5F-Apinaca indazole-based synthetic cannabinoid

🧪 Properties of 5F-AKB48

⚙️ Manufacturing Process of 5F-AKB48 (Generalized Overview)⚠️ 

General Synthetic Route (Indazole-based cannabinoids)

1. Preparation of Indazole Core
   Start with the synthesis of a 1H-indazole scaffold, typically from hydrazine derivatives and benzonitriles through a cyclization reaction.

2. Carboxylation
   The indazole core is functionalized at position 3 with a carboxylic acid group, often through Friedel–Crafts acylation or other functional group modifications.

3. Amide Coupling
   The carboxylic acid at position 3 is converted into an amide using peptide coupling reagents (e.g., EDC/HOBt or DCC) and a suitable amine such as L-valine amide or other amino acid derivatives.

4. N1-Alkylation with 5-Fluoropentyl Halide
   The nitrogen at the N1 position is alkylated using 5-fluoropentyl bromide (or a similar halide) in the presence of a base (e.g., K2CO3) in a polar aprotic solvent such as DMF.

5. Purification
   The final product is purified via column chromatography or recrystallization to achieve high purity (≥98%).

6. Characterization
   The compound is characterized by NMR, MS, IR, and HPLC to verify identity, structure, and purity.

🧪 Uses of 5F-AKB48 (5F-APINACA)⚠️

🔍 Primary Uses:

1. Pharmacological Research

   • Study of CB1 and CB2 receptor binding activity

   • Evaluation of structure-activity relationships (SAR) in synthetic cannabinoids

   • Investigation into metabolic breakdown and liver enzyme interactions

2. Forensic & Toxicological Analysis

   • Used by forensic labs to create reference standards

   • Helps in identifying and tracking new psychoactive substances (NPS) in legal or criminal investigations

3. Analytical Chemistry

   • Used as a test compound in GC-MS, LC-MS/MS, and NMR calibration and identification studies

4. Synthetic Pathway Studies

   • As a model compound for developing fluorinated analogs in medicinal chemistry

🌍 Legal Status (Summary)

• Controlled in many countries including the United States (Schedule I), the UK (Class B), Germany (NpSG), Canada (Schedule II), and others.

• Legal status may vary by region – always consult local laws before handling or purchasing.

⚠️ Important Note for Researchers and Buyers

This compound is strictly for laboratory use and must be handled by trained professionals in a certified research facility. It is not for human or veterinary consumption, inhalation, or clinical trials without proper regulatory approval.